Molecular Biology

Molecular Biology- Viva Questions- Set -1

1-State Chargaff’s rule In DNA molecules, the concentration of deoxyadenosine (A) nucleotides equals that of thymidine (T) nucleotides (A = T), while the concentration of deoxyguanosine (G) nucleotides equals that of deoxycytidine (C) nucleotides (G = C). 2- What is meant by hyperchromicity of denaturation?  The conjugated double bonds of purine and pyrimidine derivatives absorb

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Translation- an overview

The pathway of protein synthesis is called Translation because the ‘language’ of the nucleotide sequence on the mRNA is translated into the language of the amino acid sequence. The m RNA is translated from its 5’end to its 3’end, producing a protein synthesized from its amino-terminal end to its carboxyl-terminal end. Prokaryotic Translation  Components required

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Regulation Of Gene Expression in Eukaryotes

Gene regulation is significantly more complex in eukaryotes than in prokaryotes for a number of reasons- 1)  Large Genome First, the genome being regulated is significantly larger. The E. coli genome consists of a single, circular chromosome containing 4.6 Mb. This genome encodes approximately 2000 proteins. In comparison, the genome within a human cell contains 23 pairs

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Lac operon (Regulation of gene expression in prokaryotes)- lecture-2

Prokaryotes must use substances and synthesize macromolecules just fast enough to meet their needs. The genes for metabolizing enzymes are expressed only in the presence of nutrients.  If the enzymes are not needed, genes are turned off. This allows for the conservation of cell resources. Controlling gene expression is one method of regulating metabolism. Bacteria

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