Glycogen Metabolism- Multiple-Choice Questions with Complete explanations-Part-1

1. Imagine you’re preparing for a long-distance marathon and need sustained energy throughout the race. Which of the following organs stores the maximum amount of glycogen that your body can use during prolonged exercise?
A. Adipose tissue
B. Cardiac muscle
C. Kidney
D. Liver
E. Skeletal muscle

 

The correct answer is E. Skeletal muscle:
During the marathon, your skeletal muscles will provide the majority of energy needed for sustained movement. Because skeletal muscle makes up a large portion of body mass, it stores the highest total amount of glycogen in the body, which is used locally within the muscles to fuel contractions. This glycogen can keep your muscles energized over long distances, giving you the endurance you need for the race.
Other options:
A. Adipose tissue (Incorrect):
Adipose tissue, or body fat, provides a long-term energy source but primarily stores fat, not glycogen. It cannot supply the quick energy needed during intense or prolonged exercise. Instead, adipose tissue may eventually break down fat to provide energy, but this process is slower and less efficient for rapid muscle activity.
B. Cardiac muscle (Incorrect):
Your heart needs a constant energy supply to keep beating during the race, and cardiac muscle does have some glycogen stored for this purpose. However, it holds much less glycogen than skeletal muscle or the liver, as its primary function is to pump blood, not store energy.
C. Kidney (Incorrect):
Although kidneys have essential roles in maintaining blood composition and even glucose production through gluconeogenesis, they store very little glycogen compared to skeletal muscle or liver. During the marathon, their glycogen stores would be insignificant as an energy source.
D. Liver (Partially Correct):
The liver also stores a significant amount of glycogen but in a different role. Throughout the marathon, your liver can release glucose into the bloodstream to maintain your blood sugar levels, supporting energy demands across the body. However, since the liver is smaller than the entire muscle mass, it contains less total glycogen than skeletal muscle.

2. Imagine you’ve just eaten a carbohydrate-rich meal, and your body needs to store the excess glucose as glycogen. Which of the following is the active form of glucose that initiates this glycogen storage process, known as glycogenesis?
A. Glucose 1,6 Bisphosphate
B. UDP-glucose
C. UMP-Glucose
D. Glucose-6-Phosphate
E. Glucose-1-Phosphate

 

The correct answer is B. UDP-glucose: After your meal, your body begins converting excess glucose into glycogen for storage. UDP-glucose (Uridine diphosphate glucose) is the active form of glucose required to initiate glycogenesis. Once glucose is converted to UDP-glucose, it can be added to the glycogen chain, allowing your body to store energy efficiently for later use. This active form is essential because it “activates” glucose, making it ready for the glycogen synthesis process.
Other options:
A. Glucose 1,6 Bisphosphate (Incorrect):
Glucose 1,6 Bisphosphate is an intermediate in other metabolic pathways, such as glycolysis, but it is not directly involved in glycogenesis. This molecule does not serve as the active form of glucose needed to initiate glycogen storage.
C. UMP-Glucose (Incorrect):
UMP-Glucose is not a recognized molecule in glucose metabolism and does not play a role in glycogenesis.
D. Glucose-6-Phosphate (Incorrect):
Glucose-6-phosphate is a critical intermediate in several metabolic pathways. However, for glycogenesis, glucose-6-phosphate must first be converted to glucose-1-phosphate, which then forms UDP-glucose, the active form required to build glycogen.
E. Glucose-1-Phosphate (Incorrect):
Glucose-1-phosphate is a precursor in glycogenesis but is not the final active form. It must be converted to UDP-glucose before it can participate in adding glucose units to the glycogen chain.

3. Imagine a researcher is studying a glycogen storage disease caused by a defect in the branching enzyme, which prevents proper glycogen formation. Understanding the role of branching in glycogen is essential to grasp the impact of this defect. Which of the following best explains the importance of branching in glycogen structure?
A. Carried out by the glycogen synthase enzyme
B. Decreases solubility
C. Increases the rate of glycogen synthesis and degradation
D. Occurs after every 4-6 glucose residues
E. Reduces compaction

 

The correct answer is C. Increases the rate of glycogen synthesis and degradation:
Branching in glycogen’s structure provides multiple endpoints for enzymes to act upon, allowing for the rapid addition or release of glucose units. This feature is crucial for efficient glycogen storage and for quickly releasing glucose when the body needs energy. Without proper branching, glycogen is abnormal and less effective, which is why defects in the branching enzyme lead to storage diseases.
Other options:
A. Carried out by the glycogen synthase enzyme (Incorrect):
Branching is specifically performed by the branching enzyme, not glycogen synthase. Glycogen synthase only elongates glucose chains without creating branches.
B. Decreases solubility (Incorrect):
Branching actually makes glycogen more soluble, which is essential for enzyme accessibility and efficient synthesis and degradation.
D. Occurs after every 4-6 glucose residues (Incorrect):
In normal glycogen, branches typically appear after every 8-12 glucose residues, which optimizes the structure for storage and accessibility.
E. Reduces compaction (Incorrect):
Rather than reducing compaction, branching allows glycogen to be stored densely, maximizing glucose storage within cells.

4. Imagine you are studying metabolic responses under different dietary and physiological conditions. You are particularly interested in understanding the activity of glycogen synthase. In which of the following conditions do you expect glycogen synthase to be active?

A. Excess glycogen stores
B. High carbohydrate feeding
C. High-fat feeding
D. Starvation
E. Uncontrolled diabetes mellitus

The correct answer is B. High carbohydrate feeding: After a high intake of carbohydrates, blood glucose levels rise, leading to increased insulin secretion. Insulin activates glycogen synthase, promoting glycogen synthesis to store the excess glucose in the liver and muscles for future energy needs.
Other options:
A. Excess glycogen stores (Incorrect):
When glycogen stores are already full, glycogen synthase activity is generally reduced, as the body has a limited capacity for glycogen storage and does not need to produce more.
C. High-fat feeding (Incorrect):
High-fat feeding does not significantly raise blood glucose or insulin levels, so glycogen synthase remains inactive, as there is little glucose to convert into glycogen.
D. Starvation (Incorrect):
During starvation, glycogen stores are depleted, and the body focuses on gluconeogenesis (producing glucose) and glycogen breakdown rather than synthesis, so glycogen synthase is inactive.
E. Uncontrolled diabetes mellitus (Incorrect):
In uncontrolled diabetes, insulin is either absent or ineffective, leading to high blood glucose but no activation of glycogen synthase, as insulin is necessary for its activation.

5. A patient presents with symptoms of low blood sugar and muscle weakness. Laboratory tests reveal an abnormal accumulation of glycogen in liver and muscle tissues, suggesting a metabolic disorder related to glycogen degradation. As part of your investigation, you review enzymes involved in glycogen breakdown. Which of the following is not an enzyme involved in glycogen degradation?

A. Glucose-6-phosphatase
B. Phosphorylase
C. Phosphoglucomutase
D. Amylo (1-4) to (1-6) Glucan transferase
E. Amylo (1-4) to (1-4) Glucan transferase

The correct answer is E. Amylo (1-4) to (1-4) Glucan transferase:
This enzyme is not involved in glycogen degradation. Amylo (1-4) to (1-4) Glucan transferase is not a recognized enzyme in glycogen metabolism and does not contribute to glycogen breakdown.
Other options:
A. Glucose-6-phosphatase (Incorrect):
This enzyme is crucial in glycogen degradation, especially in the liver, where it converts glucose-6-phosphate into free glucose that can be released into the bloodstream to maintain blood sugar levels.
B. Phosphorylase (Incorrect):
Glycogen phosphorylase plays a central role in glycogen degradation by cleaving glucose units from the glycogen chain as glucose-1-phosphate, essential for mobilizing glucose in times of energy need.
C. Phosphoglucomutase (Incorrect):
Phosphoglucomutase converts glucose-1-phosphate to glucose-6-phosphate, which can be further processed for energy production or converted to free glucose by glucose-6-phosphatase in the liver.
D. Amylo (1-4) to (1-6) Glucan transferase (Incorrect):
Also known as the debranching enzyme, this enzyme rearranges glycogen branches, allowing phosphorylase to continue breaking down glycogen. Deficiencies in this enzyme can lead to incomplete glycogen breakdown and metabolic symptoms.

6. A patient with McArdle’s disease experiences rapid fatigue and muscle cramps during exercise. This condition limits the breakdown of glycogen in muscle, restricting the availability of glucose for energy production. In cases where glycogen breakdown is functioning normally, what would be the net energy output when glucose obtained through the action of phosphorylase on glycogen is oxidized anaerobically through glycolysis?

A. 4 ATP
B. 2 ATP
C. 3 ATP
D. 8 ATP
E. 38 ATP

 

The correct answer is C. 3 ATP: When glycogen is broken down by phosphorylase, it produces glucose-1-phosphate, which is then converted to glucose-6-phosphate. This bypasses the initial ATP-consuming step of glycolysis (where glucose is phosphorylated to glucose-6-phosphate), resulting in a net gain of 3 ATP molecules per molecule of glucose oxidized anaerobically through glycolysis.
Other Options:
A. 4 ATP (Incorrect):
Although bypassing the ATP-consuming step increases the ATP yield, the net gain is 3 ATP, not 4 ATP, per glucose derived from glycogen.
B. 2 ATP (Incorrect):
This would be the net ATP yield from glycolysis if starting with free glucose, where 1 ATP is used to convert glucose to glucose-6-phosphate.
D. 8 ATP (Incorrect):
This ATP yield is higher than what anaerobic glycolysis can produce; it may reflect the theoretical total output in aerobic conditions but not in anaerobic glycolysis.
E. 38 ATP (Incorrect):
This is the theoretical maximum yield of ATP per glucose molecule in aerobic conditions, involving both glycolysis and oxidative phosphorylation, not anaerobic glycolysis.

7. A young patient presents with low blood sugar, an enlarged liver, and high levels of lactate and uric acid in the blood. Genetic testing confirms a diagnosis of Von Gierke’s disease. A deficiency in which of the following enzymes is responsible for this condition?

A. Phosphorylase
B. Glucose-6-phosphatase
C. Debranching enzyme
D. Phosphoglucomutase
E. Branching enzyme

 

The correct answer is B. Glucose-6-phosphatase: Von Gierke’s disease (Glycogen Storage Disease Type I) is caused by a deficiency in the enzyme glucose-6-phosphatase. This enzyme is necessary for converting glucose-6-phosphate to free glucose in the liver, which can then be released into the bloodstream. Without this enzyme, glucose remains trapped in the form of glucose-6-phosphate, leading to glycogen accumulation in the liver and hypoglycemia due to impaired glucose release.
Other Options:
A. Phosphorylase (Incorrect):
Phosphorylase is responsible for breaking down glycogen into glucose-1-phosphate, but it is not directly involved in converting glucose-6-phosphate to free glucose. Deficiency in this enzyme would not cause Von Gierke’s disease.
C. Debranching enzyme (Incorrect):
The debranching enzyme helps in breaking down glycogen by removing branches, but a deficiency in this enzyme causes a different glycogen storage disease (Cori disease), not Von Gierke’s disease.
D. Phosphoglucomutase (Incorrect):
Phosphoglucomutase is involved in converting glucose-1-phosphate to glucose-6-phosphate during glycogen breakdown, but a deficiency in this enzyme does not lead to Von Gierke’s disease.
E. Branching enzyme (Incorrect):
The branching enzyme is involved in creating branches in glycogen during synthesis, and its deficiency leads to Andersen’s disease, another type of glycogen storage disorder, not Von Gierke’s disease.

8. A young athlete reports experiencing severe muscle cramps and fatigue during intense exercise, often needing to stop due to the discomfort. Lab tests reveal abnormally high levels of glycogen in the muscle tissues but normal blood glucose levels. This presentation suggests a metabolic disorder affecting glycogen breakdown, specifically in the muscles. Which of the following enzymes is deficient in this condition?

A. Hepatic hexokinase
B. Muscle phosphorylase
C. Muscle debranching enzyme
D. Muscle hexokinase
E. Muscle phosphofructokinase

 

The correct answer is B. Muscle phosphorylase: McArdle’s syndrome (Glycogen Storage Disease Type V) is caused by a deficiency in muscle glycogen phosphorylase. This enzyme is essential for breaking down glycogen in muscle cells to provide glucose for energy during intense exercise. Without this enzyme, glycogen accumulates in muscle tissue, leading to cramps and fatigue due to an inability to access stored energy efficiently.
Other Options:
A. Hepatic hexokinase (Incorrect):
Hepatic hexokinase is an enzyme involved in glucose phosphorylation in the liver, not the muscle. A deficiency in this enzyme would not result in the symptoms observed in McArdle’s syndrome.
C. Muscle debranching enzyme (Incorrect):
The muscle debranching enzyme helps remove branches in glycogen, but its deficiency leads to a different glycogen storage disease, Cori disease, and does not specifically cause McArdle’s syndrome.
D. Muscle hexokinase (Incorrect):
Muscle hexokinase is responsible for phosphorylating glucose within muscle cells, but it is not involved in glycogen breakdown. A deficiency here would not result in glycogen accumulation, as seen in McArdle’s syndrome.
E. Muscle phosphofructokinase (Incorrect):
Muscle phosphofructokinase is an enzyme in glycolysis responsible for converting fructose-6-phosphate to fructose-1,6-bisphosphate, but it does not play a direct role in glycogen breakdown. A deficiency in this enzyme causes Tarui disease, not McArdle’s syndrome.

9. A patient presents with fatigue and low endurance during high-intensity exercise. Tests reveal that while their muscles have a normal glycogen content, they experience difficulty mobilizing this glycogen effectively. Upon further investigation, you find that their muscle cells are able to release glucose-1-phosphate but seem unable to generate free glucose directly from glycogen. Which of the following enzymes is responsible for generating free glucose during glycogen breakdown in skeletal muscle?

A. Phosphorylase
B. α-1-6-amyloglucosidase
C. Debranching enzyme
D. Glucose-6-phosphatase
E. Phosphoglucomutase

 

The correct answer is B. α-1-6-amyloglucosidase: The α-1-6-amyloglucosidase activity, part of the debranching enzyme complex, is responsible for releasing free glucose from the branch points (α-1,6 linkages) in glycogen. This free glucose can be quickly used within the muscle cells for energy during high-intensity activity.
Other Options:
A. Phosphorylase (Incorrect):
Glycogen phosphorylase removes glucose units from glycogen in the form of glucose-1-phosphate, not free glucose. It works on the linear α-1,4 linkages but does not release glucose directly.
C. Debranching enzyme (Incorrect, partially correct):
While the debranching enzyme includes both transferase and α-1-6-amyloglucosidase activities, only the α-1-6-amyloglucosidase component releases free glucose.
D. Glucose-6-phosphatase (Incorrect):
Glucose-6-phosphatase, which converts glucose-6-phosphate to free glucose, is absent in skeletal muscle and is only present in the liver. Muscle cells cannot release free glucose into the bloodstream.
E. Phosphoglucomutase (Incorrect):
Phosphoglucomutase converts glucose-1-phosphate to glucose-6-phosphate in muscle cells but does not release free glucose.

10. A patient with hypoglycemia reports experiencing low energy levels, especially during fasting periods or intense exercise. Laboratory tests indicate normal glycogen levels in the muscle but an inability to release free glucose into the bloodstream from muscle glycogen stores. This suggests that skeletal muscle is missing a specific enzyme that is essential for releasing glucose into circulation. Which of the following enzymes of glycogen metabolism is absent in skeletal muscle?

A. Phosphorylase
B. α-1-6-amyloglucosidase
C. Debranching enzyme
D. Glucose-6-phosphatase
E. Phosphoglucomutase

The correct answer is D. Glucose-6-phosphatase: Glucose-6-phosphatase is absent in skeletal muscle. This enzyme converts glucose-6-phosphate to free glucose, a step required for releasing glucose into the bloodstream. Because skeletal muscle lacks this enzyme, it cannot release glucose into circulation and instead uses glucose-6-phosphate for its own energy needs.
Other Options:
A. Phosphorylase (Incorrect):
Phosphorylase is present in skeletal muscle and breaks down glycogen into glucose-1-phosphate, enabling further processing for muscle energy use.
B. α-1-6-amyloglucosidase (Incorrect):
α-1-6-amyloglucosidase, part of the debranching enzyme complex, is present in muscle and helps remove glycogen branches for efficient glycogen breakdown.
C. Debranching enzyme (Incorrect):
The debranching enzyme is also present in skeletal muscle and aids in glycogen degradation by facilitating the removal of branches.
E. Phosphoglucomutase (Incorrect):
Phosphoglucomutase, which converts glucose-1-phosphate to glucose-6-phosphate, is active in muscle cells to support energy production.

 

error: Content is protected !!